Sunil Madhogarhia is a SUNIL MADHOGARHIA based in KOLKATA, India. Sunil Madhogarhia is a member of exporthub.com since August, 2006. Our business is related to Beauty & Personal Care industry and we specifically deal in DIAGNOSTIC REAGENTS, PP BAGS, JUTE BAGS. Please find our product details below:REQUIRED : DISTRIBUTOR for USA made ANTIGEN based TB Test in INDIAM. tuberculosis Antigen ELISAFor the detection and quantitative determination of specific antigen of M. tuberculosis secreted in culture broth, human blood or pleural effusion. QUICK INSTRUCTION:Preparation:1.Bring all reagents and samples to room temperature (21-25℃).2.Prepare 1x Washing Solution by diluting 20x concentrated Washing Solution with reagent grade water.3.Thoroughly mix all the reagents in the kit and samples . Test Procedure: Set enough strips on the strip holder ↓Apply 100 ul of PCs, NC and samples to wells 100 ul ↓ Incubate at 37℃ for 60 min 60 min ↓Aspirate all liquid from wells. Wash for 3 times by 280-300 ul Washing Solution WASH ↓Add 100 ul of Conjugate to wells 100 ul ↓Incubate at 37℃ for 30 min 30 min ↓Aspirate all liquid from wells. Wash wells for3 times by280-300 ul Washing Solution WASH ↓Add 100 ul of Substrate (TMB) to wells 100 ul ↓ Incubate at 37℃ for 10 min 10 min ↓ Add 100 ul of Stop Solution (1N HCI) to wells 100 ul ↓Read assay plate at 450 nm using 650 nm as reference OD450/650Quick and Sure Micro Check M. tuberculosis Antigen ELISAINTENDED USE: The Quick and Sure Micro Check xx Antigen ELSA, is an enzyme linked immunsorbent assay intended for the detection quantitative determination of specific antigen of M. tuberculosis secreted in culture brothThank you for taking the time to go through our business profile. If there are any question, inquiry or comments, please feel free to contact us.